Closing Thoughts

The forest through the trees.

Prerequisites for bioinformatics

• Access to a multi-core (24 cpu or greater), ‘high’ memory 64Gb or greater Linux server.
• Familiarity with the ’command line’ and at least one programming language.
• Basic knowledge of how to install software
• Basic knowledge of R (or equivalent) and statistical programming
• Basic knowledge of Statistics and model building

The bottom line

• Spend the time (and money) planning and producing good quality, accurate and sufficient data for your experiment.
• Get to know to your data, develop and test expectations
• Result, you’ll spend much less time (and less money) extracting biological significance and results during analysis.

Next Steps

My recommendation is to follow all of the instructions again, from the beginning on your own. Then again if needed, this time making changes and edits to the code to better understand things.